|Monomer||Entacmaea quadricolor||26.1 kDa||-|
|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
|% Normal Cells||OSER/NE ratio||Cell Type||Reference|
|57.7 ± 7.5 (10000 cells)||-||HeLa||Cranfill et al. (2016)|
|t1/2 (s)||Power||Light||Mode||In Cell||Fusion||˚C||Reference|
|48.0||Merzlyak et al. (2007)|
TagRFP was derived from TurboRFP with the following mutations: K42R/K67R/A68T/I93V/F110L/N112D/I115L/N122R/R138L/R155E/H157R/Q159D/Y169H/H171I/S173N/F192V/H193Y/F194Y/M216V/K220R/R231K
Note: While the sequence below matches the sequence shown in Supp. Fig 1 of the original publication (Merzlyak et al, 2007), most commonly used variants (e.g. at Addgene and Snapgene) show TagRFP with N terminus from EGFP (MVSKGEE). Another literature inconsistency: Subach et al (2008), which derives mTagBFP from TagRFP, shows TagRFP with two glutamic acids in the N terminus instead of one.
No excerpts have been added for TagRFP
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).