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Comparison List

DsRed2

DsRed2 is a basic (constitutively fluorescent) red fluorescent protein published in 2002, derived from Discosoma sp.. It is reported to be a very slowly-maturing tetramer.
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Aggregation Organism Molecular Weight Cofactor
Tetramer Discosoma sp. 25.8 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
561 587 52,000 0.55 28.6   390.0  

OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
- (10000 cells) - HeLa Cranfill et al. (2016)

Photostability

no photostability measurements available ... add one!

DsRed2 Sequence

DsRed2 was derived from DsRed with the following mutations: R2A/K5E/K9T/V105A/I161T/S197A

MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVKLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHHLFL
GenBank: ABS86946
IPG: 13508869

Excerpts

No excerpts have been added for DsRed2
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed)

Bevis Bj, Glick Bs

(2002). Nature Biotechnology, 20(1) , 83-87. doi: 10.1038/nbt0102-83. Article   Pubmed

Additional References

  1. Two-photon absorption properties of fluorescent proteins

    Drobizhev M, Makarov Ns, Tillo Se, Hughes Te, Rebane A

    (2011). Nature Methods, 8(5) , 393-399. doi: 10.1038/nmeth.1596. Article   Pubmed

External Resources

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