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Comparison List

mCherry2

mCherry2 is a basic (constitutively fluorescent) red fluorescent protein published in 2017, derived from Discosoma sp.. It has very low acid sensitivity.
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Aggregation Organism Molecular Weight Cofactor
Monomer Discosoma sp. 26.7 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
589 610 79,400 0.22 17.47 3.3    

Photostability

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mCherry2 Sequence

mCherry2 was derived from mCherry with the following mutations: K92N/K138C/K139R/S147T/N196D/T202L
amino acid numbers relative to DsRed. show relative to mCherry

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERAEGRHSTGGMDELYK

Excerpts

mFruit variant mCherry2 is a further engineered variant of mCherry that retains similar excitation and emission maxima (λex = 589 nm and λem = 610 nm) but has slightly higher brightness. This variant was generated some years ago by several rounds of directed evolution of an mCherry-ferritin fusion, as was originally described for Superfolder GFP (Waldo et al, 1999), but was not characterized in detail until the current work

Shen et al. (2017)

Primary Reference

Additional References

External Resources

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