Comparison List


mCherry2 is a basic (constitutively fluorescent) red fluorescent protein published in 2017, derived from Discosoma sp.. It has very low acid sensitivity.
Oligomerization Organism Molecular Weight Cofactor
Monomer Discosoma sp. 26.7 kDa -

FPbase ID: CN92E


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
589 610 79,400 0.22 17.47 3.3    


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mCherry2 Sequence

mCherry2 was derived from mCherry with the following mutations: K92N/K138C/K139R/S147T/N196D/T202L
amino acid numbers relative to DsRed. show relative to mCherry



mFruit variant mCherry2 is a further engineered variant of mCherry that retains similar excitation and emission maxima (λex = 589 nm and λem = 610 nm) but has slightly higher brightness. This variant was generated some years ago by several rounds of directed evolution of an mCherry-ferritin fusion, as was originally described for Superfolder GFP (Waldo et al, 1999), but was not characterized in detail until the current work

Shen et al. (2017)

Primary Reference

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