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Comparison List

mRuby3

mRuby3 is a basic (constitutively fluorescent) red fluorescent protein published in 2016, derived from Entacmaea quadricolor. It has low acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Entacmaea quadricolor 26.6 kDa -

FPbase ID: MZMFB

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
558 592 128,000 0.45 57.6 4.8 136.5  

mRuby3 OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
14.0 (70 cells) - U-2 OS Bindels et al. (2016)

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
349.0   Bajar et al. (2016)

mRuby3 Sequence

mRuby3 was derived from mRuby2 with the following mutations: N33R/M36E/T38V/K74A/G75D/M105T/C114E/H118N/Q120K/H159D/M160I/S171H/S173N/I192V/L202I/M209T/F210Y/H216V/F221Y/A222S/G223N
amino acid numbers relative to eqFP611. show relative to mRuby2

MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTMRIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGGVVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTDIALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAVAKYSNLGGGMDELYK
GenBank: ATE88097

Excerpts

Negligible photochromic behavior was measured for the mScarlet variants, while TagRFP-T, mRuby2, mRuby3 and mApple showed 15%, 19%, 41%, and 51% photochromic behavior, respectively. Hence, extreme care must be taken when using the latter four RFP variants as acceptors in FRET studies, since a photochromic effect is easily confused with a changed FRET state, especially if one considers that the typical FRET contrast in many sensors is in the range of only 5–20%. The photochromic behavior can also interfere with characterization of FPs, like determination of photostability (Supplementary Fig. 8) or brightness (Supplementary Fig. 5l).

Bindels et al. (2016)

Primary Reference

Additional References

External Resources

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