| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | Entacmaea quadricolor | 26.6 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 558 | 592 | 128,000 | 0.45 | 57.6 | 4.8 | 136.5 |
| % Normal Cells | OSER/NE ratio | Cell Type | Reference |
|---|---|---|---|
| 14.0 (70 cells) | - | U-2 OS | Bindels et al. (2016) |
| t1/2 (s) | Power | Light | Mode | In Cell | Fusion | ˚C | Reference |
|---|---|---|---|---|---|---|---|
| 349.0 | Bajar et al. (2016) |
mRuby3 was derived from mRuby2 with the following mutations: N33R/M36E/T38V/K74A/G75D/M105T/C114E/H118N/Q120K/H159D/M160I/S171H/S173N/I192V/L202I/M209T/F210Y/H216V/F221Y/A222S/G223N
amino acid numbers relative to eqFP611. show relative to mRuby2
Negligible photochromic behavior was measured for the mScarlet variants, while TagRFP-T, mRuby2, mRuby3 and mApple showed 15%, 19%, 41%, and 51% photochromic behavior, respectively. Hence, extreme care must be taken when using the latter four RFP variants as acceptors in FRET studies, since a photochromic effect is easily confused with a changed FRET state, especially if one considers that the typical FRET contrast in many sensors is in the range of only 5–20%. The photochromic behavior can also interfere with characterization of FPs, like determination of photostability (Supplementary Fig. 8) or brightness (Supplementary Fig. 5l).
Bindels et al. (2016)
(2016). Scientific Reports, 6(1) , 20889. doi: 10.1038/srep20889. Article Pubmed
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(2017). Nature Methods, 15(1) , 47-51. doi: 10.1038/nmeth.4509. Article Pubmed
(2016). Nature Methods, 14(1) , 53-56. doi: 10.1038/nmeth.4074. Article Pubmed
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