Oligomerization | Organism | Molecular Weight | Cofactor |
---|---|---|---|
Monomer | synthetic construct | 26.4 kDa | - |
Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
---|---|---|---|---|---|---|---|
569 | 593 | 104,000 | 0.54 | 56.16 | 5.4 | 36.0 | 3.1 |
% Normal Cells | OSER/NE ratio | Cell Type | Reference |
---|---|---|---|
76.0 (199 cells) | 1.4 ± 0.2 (23 cells) | U-2 OS | Bindels et al. (2016) |
t1/2 (s) | Power | Light | Mode | In Cell | Fusion | ˚C | Reference |
---|---|---|---|---|---|---|---|
225.0 | 6.9 (W/cm2) | Arc-lamp | Widefield | H2A | 37.0 | Bindels et al. (2016) | |
190.0 | 1.35 (W/cm2) | Laser | Spinning Disc Confocal | H2A | 37.0 | Bindels et al. (2016) |
mScarlet-I was derived from mScarlet with the following mutations: T74I
The single amino acid substitution T74I found in mScarlet-I results in a marked maturation acceleration in cells, but at the cost of a moderate decrease in fluorescence quantum yield (0.54) and fluorescence lifetime (3.1 ns), although both values are still higher than those of all previously engineered bright mRFPs.
Bindels et al. (2016)
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