Optimal fluorescent protein tags for quantifying protein oligomerization in living cells

Dunsing V, Luckner M, Zühlke B, Petazzi Ra, Herrmann A, Chiantia S

(2018). Scientific Reports, 8(1) , 10634. doi: 10.1038/s41598-018-28858-0. Article   Pubmed

    Secondary Proteins:
  1. mCardinal,
  2. mCherry,
  3. mCherry2,
  4. mEGFP,
  5. mEYFP,
  6. mRuby3,
  7. mScarlet,
  8. mScarlet-I
Add photostability measurements

Excerpts

mCherry2, a not fully characterized mCherry variant, possesses superior properties compared to all other tested red FPs, i.e. mCherry, mCardinal, mRuby3, mScarlet and mScarlet-I. Additionally, by performing Fluorescence Cross-Correlation Spectroscopy (FCCS) measurements of FP hetero-dimers, we show that mCherry2 improves the quantification of the spectral cross-correlation compared to mCherry and propose to use mEGFP and mCherry2 as a novel standard FP pair for hetero-interaction studies.