|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
|% Normal Cells||OSER/NE ratio||Cell Type||Reference|
|80.0 (215 cells)||1.7 ± 0.3 (41 cells)||U-2 OS||Bindels et al. (2016)|
|t1/2 (s)||Power||Light||Mode||In Cell||Fusion||˚C||Reference|
|277.0||6.9 (W/cm2)||Arc-lamp||Widefield||H2A||37.0||Bindels et al. (2016)|
|161.0||1.35 (W/cm2)||Laser||Spinning Disc Confocal||H2A||37.0||Bindels et al. (2016)|
mScarlet was derived from mRed7Q1S1BM with the following mutations: F84Y/V176A/E199K
The fluorescence lifetime of mScarlet is 3.9 ns, the highest value recorded to date for mRFPs, and it shows monoexponential decay. The quantum yield of mScarlet is 0.70; much higher than the quantum yield of other monomeric RFPs. The extinction coefficient of mScarlet is 100,300 M−1 cm−1, resulting in the highest calculated brightness in the mRFP spectral class, with a >3.5-fold increase relative to mCherry. Brightness analysis in mammalian cells confirmed that mScarlet is an extremely bright mRFP , especially when compared with mRuby2, mRuby3 and TagRFP-T.