Primary Proteins:
  1. mRed7
  2. mRed7Q1
  3. mRed7Q1S1
  4. mRed7Q1S1BM
  5. mScarlet
  6. mScarlet-H
  7. mScarlet-I
    Secondary Proteins:
  1. mApple,
  2. mKate2,
  3. mRuby2,
  4. TagRFP-T

Bleach Measurements

Protein (state) t1/2 (s) Power Light Mode In Cell Fusion ˚C
TagRFP-T 337.0
mApple 4.8
mKate2 84.0
mRuby2 123.0
mScarlet 277.0 6.9 W/cm2 Arc-lamp Widefield H2A 37.0
mScarlet 161.0 1.35 W/cm2 Laser Spinning Disc Confocal H2A 37.0
mScarlet-I 225.0 6.9 W/cm2 Arc-lamp Widefield H2A 37.0
mScarlet-I 190.0 1.35 W/cm2 Laser Spinning Disc Confocal H2A 37.0
mScarlet-H 574.0 6.9 W/cm2 Arc-lamp Widefield H2A 37.0
mScarlet-H 368.0 1.35 W/cm2 Laser Spinning Disc Confocal H2A 37.0
A caution on interpretation of photostability measurements
Add photostability measurements

OSER Measurements

Protein % Normal Cells OSER/NE ratio Cell Type Temp (˚C)
mScarlet 80.0 (215 cells) 1.7 ± 0.3 (41 cells) U-2 OS -
mScarlet-I 76.0 (199 cells) 1.4 ± 0.2 (23 cells) U-2 OS -
mScarlet-H 82.0 (284 cells) 2.2 ± 0.2 (33 cells) U-2 OS -
dTomato 31.0 (258 cells) 5.7 ± 0.7 (110 cells) U-2 OS -
SGFP2 77.0 (104 cells) 1.7 ± 0.2 (40 cells) U-2 OS -
mCherry 80.0 (369 cells) 2.0 ± 0.3 (38 cells) U-2 OS -
mApple 87.0 (103 cells) 2.2 ± 0.2 (14 cells) U-2 OS -
mKate2 59.0 (102 cells) 3.6 ± 0.8 (42 cells) U-2 OS -
mRuby2 14.0 (113 cells) - U-2 OS -
mRuby3 14.0 (70 cells) - U-2 OS -