|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
|% Normal Cells||OSER/NE ratio||Cell Type||Reference|
|82.0 (284 cells)||2.2 ± 0.2 (33 cells)||U-2 OS||Bindels et al. (2016)|
|t1/2 (s)||Power||Light||Mode||In Cell||Fusion||˚C||Reference|
|574.0||6.9 (W/cm2)||Arc-lamp||Widefield||H2A||37.0||Bindels et al. (2016)|
|368.0||1.35 (W/cm2)||Laser||Spinning Disc Confocal||H2A||37.0||Bindels et al. (2016)|
mScarlet-H was derived from mScarlet with the following mutations: M164H
Screening for a more photostable mScarlet variant yielded mScarlet-H, with a single amino acid substitution (M164H) causing a >2-fold improvement in photostability, albeit accompanied by a serious decrease in quantum yield and fluorescence lifetime.