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IrisFP-M159A

IrisFP-M159A is a photoswitchable green fluorescent protein published in 2015, derived from Lobophyllia hemprichii. It has low acid sensitivity.

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Oligomerization	Organism	Molecular Weight	Cofactor
Tetramer	Lobophyllia hemprichii	25.6 kDa	-

FPbase ID: T44HU

Attributes

State	Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
Green	484	513	62,800	0.18	11.3	4.7
Green (off)

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Transitions

From	To	Switch λ
Green (off)	Green	405
Green	Green (off)	488

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Photostability

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IrisFP-M159A Sequence

BLAST

IrisFP-M159A was derived from IrisFP with the following mutations: M159AM159A

MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKEGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARNDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDITAALLLEGNAHYRCDSRTTYKAKEKGVKLPGYHLVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDNARR

Structure

PDB ID

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for IrisFP-M159A
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Rational design of enhanced photoresistance in a photoswitchable fluorescent protein

Duan C, Byrdin M, El Khatib M, Henry X, Adam V, Bourgeois D

(2015). Methods and Applications in Fluorescence, 3(1) , 014004. doi: 10.1088/2050-6120/3/1/014004. Article Pubmed

Additional References

No additional references have been added.

External Resources

Search Addgene for IrisFP-M159A
Search RIKEN BRC for IrisFP-M159A

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