a.k.a. SPOntaneous switching ON fluorescent protein
| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | Aequorea victoria | 26.8 kDa | - |
| State | Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|---|
| on | 510 | 527 | 54,000 | 0.5 | 27.0 | 7.2 | 112.0 | |
| off |
No photostability measurements available ... add one!
SPOON was derived from Dreiklang with the following mutations: I47V/T59S/M153T/S208G/M233T
amino acid numbers relative to avGFP. show relative to Dreiklang
Dreiklang can switch on spontaneously by thermal relaxation that is caused by a dehydration of chromophore, which enables a simpler single molecule localization-based microscopy, called DSSM (decoupled stochastic switching microscopy)... Compared to GSDIM, DSSM enables super-resolution imaging at relatively lower power density illumination for switching-off (0.2 kW cm–2 by 405 nm light, while GSDIM required 3 kW cm–2). However, DSSM still requires 405 nm illumination to make Dreiklang adopt the fluorescent off state. To overcome this problem, we developed an improved PSFP (SPOON) that has a fast blinking behavior due to thermal switching-on and rapid photoswitching-off with the same 488 nm excitation light needed for fluorescent emission.
Arai et al. (2018)
(2018). ACS Chemical Biology, , . doi: 10.1021/acschembio.8b00200. Article Pubmed
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