Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology

Liljeruhm J, Funk Sk, Tietscher S, Edlund Ad, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynnå A, Ivermark K, Lövgren J, Törnblom V, Virtanen A, Lundin Er, Wistrand-Yuen E, Forster Ac

(2018). Journal of Biological Engineering, 12(1) , . doi: 10.1186/s13036-018-0100-0. Article

    Primary Proteins:
  1. amilCP-Orange
  2. amilCP-Pink
  3. fwYellow
  4. scOrange
  5. tsPurple
    Secondary Proteins:
  1. aeBlue,
  2. amFP486,
  3. amilCP,
  4. amilGFP,
  5. asulCP,
  6. cjBlue,
  7. eforCP,
  8. gfasCP,
  9. meffRFP,
  10. Rtms5,
  11. spisCP
Add photostability measurements

Excerpts

Interestingly, one CP, aeBlue, changed color over time (Fig. 1c), indicating multi-step maturation.