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amFP486

a.k.a. amajGFP, amajCFP, amajLime

amFP486 is a basic (constitutively fluorescent) cyan fluorescent protein published in 1999, derived from Anemonia majano.
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Oligomerization Organism Molecular Weight Cofactor
Tetramer Anemonia majano 25.3 kDa -

FPbase ID: 1M48O

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
458 486 40,000 0.24 9.6      
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Photostability

No photostability measurements available ... add one!

amFP486 Sequence

BLAST
MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGNGKPYEGTQTSTFKVTMANGGPLAFSFDILSTVFKYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDGGVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKKTTGWDPSFEKMTVCDGILKGDVTAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHVVEHRIARTDLDKGGNSVQLTEHAVAHITSVVPF
GenBank: AAF03371
UniProtKB: Q9U6Y6
IPG: 1173879

Structure

Deposited: ,
Chromophore:

Excerpts

Table 1: Fluorescence quantum yield of amFP486: 0.75 (0.24 (Matz et al., 1999), 0.71 (Henderson et al., 2005))

Molina et al. (2017)

Primary Reference

Erratum: Fluorescent proteins from nonbioluminescent Anthozoa species

Matz Mv, Fradkov Af, Labas Ya, Savitsky Ap, Zaraisky Ag, Markelov Ml, Lukyanov Sa

(1999). Nature Biotechnology, 17(10) , 969-973. doi: 10.1038/13657. Article   Pubmed

Additional References

  1. Overcoming chromoprotein limitations by engineering a red fluorescent protein

    Bao L, Menon Pnk, Liljeruhm J, Forster Ac

    (2020). Analytical Biochemistry, 611, 113936. doi: 10.1016/j.ab.2020.113936. Article   Pubmed

  2. Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology

    Liljeruhm J, Funk Sk, Tietscher S, Edlund Ad, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynnå A, Ivermark K, Lövgren J, Törnblom V, Virtanen A, Lundin Er, Wistrand-Yuen E, Forster Ac

    (2018). Journal of Biological Engineering, 12(1) , . doi: 10.1186/s13036-018-0100-0. Article

  3. Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation

    Molina Rs, Tran Tm, Campbell Re, Lambert Gg, Salih A, Shaner Nc, Hughes Te, Drobizhev M

    (2017). The Journal of Physical Chemistry Letters, 8(12) , 2548-2554. doi: 10.1021/acs.jpclett.7b00960. Article   Pubmed

  4. Spectral Diversity of Fluorescent Proteins from the Anthozoan Corynactis californica

    Schnitzler Ce, Keenan Rj, Mccord R, Matysik A, Christianson Lm, Haddock Shd

    (2008). Marine Biotechnology, 10(3) , 328-342. doi: 10.1007/s10126-007-9072-7. Article   Pubmed

  5. Crystal structures and mutational analysis of amFP486, a cyan fluorescent protein from Anemonia majano

    Henderson Jn, Remington Sj

    (2005). Proceedings of the National Academy of Sciences, 102(36) , 12712-12717. doi: 10.1073/pnas.0502250102. Article   Pubmed

  6. Diversity and evolution of the green fluorescent protein family

    Labas Ya, Gurskaya Ng, Yanushevich Yg, Fradkov Af, Lukyanov Ka, Lukyanov Sa, Matz Mv

    (2002). Proceedings of the National Academy of Sciences, 99(7) , 4256-4261. doi: 10.1073/pnas.062552299. Article   Pubmed

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