Comparison List


a.k.a. asCP, asCP595, asFP595, asPink, FP595

asulCP is a basic (constitutively fluorescent) red fluorescent protein published in 2000, derived from Anemonia sulcata.
Oligomerization Organism Molecular Weight Cofactor
? Anemonia sulcata 25.9 kDa -

FPbase ID: M3FBS


Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
572 595 120,000 0.001 0.12      


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asulCP Sequence

GenBank: AAG02385
UniProtKB: Q9GZ28
IPG: 514601


Deposited: ,
Chromophore (MYG):


The fluorescence of asFP595 is extremely weak (quantum yield , 0.001); nevertheless, the purple protein can be detected by spectrofluorimetry (Fig. 3B). When viewed by fluorescence microscopy, the novel protein shows an unexpected feature; although the fluorescence at 595 nm is virtually imperceptible at the start of observation, the emission intensity increases dramatically following a 10 –20-s exposure to green light (Fig. 4B). This effect is reversible because in the absence of incident green light, the fluorescence capacity slowly decreases to the basal level. Even more surprisingly, asFP595 fluorescence can be quenched by a flash of blue light (Fig. 4C). This effect strongly depends on intensity of irradiation: the brighter the light the more pronounced increasing and quenching of the fluorescence.

Lukyanov et al. (2000)

Extinction coefficient ~120,000 M-1 cm-1 according to our refined data. Previous studies showed a lower extinction coefficient, probably due to the incomplete protein maturation (1).

Chudakov et al. (2003)

Primary Reference

Additional References

  1. Overcoming chromoprotein limitations by engineering a red fluorescent protein

    Bao L, Menon Pnk, Liljeruhm J, Forster Ac

    (2020). Analytical Biochemistry, 611, 113936. doi: 10.1016/j.ab.2020.113936. Article   Pubmed

  2. Chromophore Environment Provides Clue to “Kindling Fluorescent Protein” Riddle

    Chudakov Dm, Feofanov Av, Mudrik Nn, Lukyanov S, Lukyanov Ka

    (2003). Journal of Biological Chemistry, 278(9) , 7215-7219. doi: 10.1074/jbc.m211988200. Article

  3. Diversity and evolution of the green fluorescent protein family

    Labas Ya, Gurskaya Ng, Yanushevich Yg, Fradkov Af, Lukyanov Ka, Lukyanov Sa, Matz Mv

    (2002). Proceedings of the National Academy of Sciences, 99(7) , 4256-4261. doi: 10.1073/pnas.062552299. Article   Pubmed

  4. Alternative Cyclization in GFP-like Proteins Family

    Martynov Vi, Savitsky Ap, Martynova Ny, Savitsky Pa, Lukyanov Ka, Lukyanov Sa

    (2001). Journal of Biological Chemistry, 276(24) , 21012-21016. doi: 10.1074/jbc.m100500200. Article

  5. Cracks in the beta -can: Fluorescent proteins from Anemonia sulcata (Anthozoa, Actinaria)

    Wiedenmann J, Elke C, Spindler K-D, Funke W

    (2000). Proceedings of the National Academy of Sciences, 97(26) , 14091-14096. doi: 10.1073/pnas.97.26.14091. Article   Pubmed

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