| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Monomer | Entacmaea quadricolor | 27.6 kDa | - |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 555 | 584 | 81,000 | 0.41 | 33.21 | 4.6 | 100.0 | 2.3 |
| % Normal Cells | OSER/NE ratio | Cell Type | Reference |
|---|---|---|---|
| 41.2 ± 4.0 (10000 cells) | - | HeLa | Cranfill et al. (2016) |
| 43.0 (740 cells) | - | U-2 OS | Manna et al. (2018) |
| t1/2 (s) | Power | Light | Mode | In Cell | Fusion | ˚C | Reference |
|---|---|---|---|---|---|---|---|
| 337.0 | Bindels et al. (2016) |
TagRFP-T was derived from TagRFP with the following mutations: S2_S2delinsVSKGE/S158T/H230_K231insKLNGMDELY
Note: While the only significant mutation in TagRFP-T relative to the parental TagRFP is S158T, here we show the version with the addition of the EGFP N- and C- termini that is by far the most variant (e.g. on Addgene and SnapGene)
Negligible photochromic behavior was measured for the mScarlet variants, while TagRFP-T, mRuby2, mRuby3 and mApple showed 15%, 19%, 41%, and 51% photochromic behavior, respectively. Hence, extreme care must be taken when using the latter four RFP variants as acceptors in FRET studies, since a photochromic effect is easily confused with a changed FRET state, especially if one considers that the typical FRET contrast in many sensors is in the range of only 5–20%. The photochromic behavior can also interfere with characterization of FPs, like determination of photostability (Supplementary Fig. 8) or brightness (Supplementary Fig. 5l).
Bindels et al. (2016)
(2008). Nature Methods, 5(6) , 545-551. doi: 10.1038/nmeth.1209. Article Pubmed
(2016). Nature Methods, 14(1) , 53-56. doi: 10.1038/nmeth.4074. Article Pubmed
(2016). Molecular Biology of the Cell, 27(22) , 3385-3394. doi: 10.1091/mbc.e16-01-0063. Article Pubmed
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