Directed evolution of excited state lifetime and brightness in FusionRed using a microfluidic sorter

Manna P, Hung S-T, Mukherjee S, Friis P, Simpson Dm, Lo Mn, Palmer Ae, Jimenez R

(2018). Integrative Biology, 10(9) , 516-526. doi: 10.1039/c8ib00103k. Article   Pubmed

    Primary Proteins:
  1. FR-1
  2. FusionRed-M
    Secondary Proteins:
  1. FusionRed
Add photostability measurements

OSER Measurements

Protein % Normal Cells OSER/NE ratio Cell Type Temp (˚C)
TagRFP-T 43.0 (740 cells) - U-2 OS -
FusionRed 86.0 (1220 cells) - U-2 OS -
FusionRed-M 87.0 (500 cells) - U-2 OS -
mCherry 69.0 (1130 cells) - U-2 OS -

Excerpts

No excerpts have been added from this reference.
Excerpts are snippets from publications that capture key information about a protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).