Ralph Jimenez

1. High-Speed Multiparameter Photophysical Analyses of Fluorophore Libraries

   Dean Km, Davis Lm, Lubbeck Jl, Manna P, Friis P, Palmer Ae, Jimenez R

   (2015). Analytical Chemistry, 87(10) , 5026-5030. doi: 10.1021/acs.analchem.5b00607. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. mOrange2

2. Far-Red Emission of mPlum Fluorescent Protein Results from Excited-State Interconversion between Chromophore Hydrogen-Bonding States

   Yoon E, Konold Pe, Lee J, Joo T, Jimenez R

   (2016). The Journal of Physical Chemistry Letters, 7(12) , 2170-2174. doi: 10.1021/acs.jpclett.6b00823. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. mPlum

3. Fluorescence from Multiple Chromophore Hydrogen-Bonding States in the Far-Red Protein TagRFP675

   Konold Pe, Yoon E, Lee J, Allen Sl, Chapagain Pp, Gerstman Bs, Regmi Ck, Piatkevich Kd, Verkhusha Vv, Joo T, Jimenez R

   (2016). The Journal of Physical Chemistry Letters, 7(15) , 3046-3051. doi: 10.1021/acs.jpclett.6b01172. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. TagRFP675

4. Directed evolution of excited state lifetime and brightness in FusionRed using a microfluidic sorter

   Manna P, Hung S-T, Mukherjee S, Friis P, Simpson Dm, Lo Mn, Palmer Ae, Jimenez R

   (2018). Integrative Biology, 10(9) , 516-526. doi: 10.1039/c8ib00103k. Article   Pubmed

   Primary Proteins:

   1. FR-1
   2. FusionRed-M

   Additional Proteins:

   1. FusionRed

5. Erratum: Directed evolution of excited state lifetime and brightness in FusionRed using a microfluidic sorter

   Manna P, Hung S-T, Mukherjee S, Friis P, Simpson Dm, Lo M, Palmer Ae, Jimenez R

   (2019). Integrative Biology, , . doi: 10.1093/intbio/zyz032. Article   Pubmed

   Primary Proteins:

   Additional Proteins:

   1. FusionRed-M

6. Engineering of a Brighter Variant of the FusionRed Fluorescent Protein Using Lifetime Flow Cytometry and Structure-Guided Mutations

   Mukherjee S, Hung S-T, Douglas N, Manna P, Thomas C, Ekrem A, Palmer Ae, Jimenez R

   (2020). Biochemistry, 59(39) , 3669-3682. doi: 10.1021/acs.biochem.0c00484. Article   Pubmed

   Primary Proteins:

   1. FusionRed-MQV

7. Directed evolution of a bright variant of mCherry: Suppression of non-radiative decay by fluorescence lifetime selections

   Mukherjee S, Manna P, Hung S-T, Vietmeyer F, Friis P, Palmer A, Jimenez R

   (2022). , , . doi: 10.26434/chemrxiv-2022-v3t03. Article

   Primary Proteins:

   1. mCherry-XL

8. Directed Evolution of a Bright Variant of mCherry: Suppression of Nonradiative Decay by Fluorescence Lifetime Selections

   Mukherjee S, Manna P, Hung S-T, Vietmeyer F, Friis P, Palmer Ae, Jimenez R

   (2022). The Journal of Physical Chemistry B, , . doi: 10.1021/acs.jpcb.2c01956. Article

   Primary Proteins:

   Additional Proteins:

   1. mCherry-XL