Engineering of a Brighter Variant of the FusionRed Fluorescent Protein Using Lifetime Flow Cytometry and Structure-Guided Mutations

Mukherjee S, Hung S-T, Douglas N, Manna P, Thomas C, Ekrem A, Palmer Ae, Jimenez R

(2020). Biochemistry, 59(39) , 3669-3682. doi: 10.1021/acs.biochem.0c00484. Article   Pubmed

    Primary Proteins:
  1. FusionRed-MQV

Bleach Measurements

Protein (state) t1/2 (s) Power Light Mode In Cell Fusion ˚C
FusionRed-MQV 38.0 5.0 W/cm2 LED Widefield 37.0
A caution on interpretation of photostability measurements
Add photostability measurements

Excerpts

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