Directed evolution of a bright variant of mCherry: Suppression of non-radiative decay by fluorescence lifetime selections

Mukherjee S, Manna P, Hung S-T, Vietmeyer F, Friis P, Palmer A, Jimenez R

(2022). , , . doi: 10.26434/chemrxiv-2022-v3t03. Article

    Primary Proteins:
  1. mCherry-XL

Bleach Measurements

Protein (state) t1/2 (s) Power Light Mode In Cell Fusion ˚C
mCherry-XL (Fluorescent) 55.0 10.0 W/cm2 LED Widefield 25.0
A caution on interpretation of photostability measurements
Add photostability measurements

Excerpts

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