|Protein (state)||t1/2 (s)||Power||Light||Mode||In Cell||Fusion||˚C|
We created monomeric optimized variants of ECFP and EYFP, which fold faster and more efficiently at 37°C and have superior solubility and brightness. Bacteria expressing SCFP3A were 9-fold brighter than those expressing ECFP and 1.2-fold brighter than bacteria expressing Cerulean. In HeLa cells, the improvements were less pronounced; nonetheless, cells expressing SCFP3A and SYFP2 were both 1.5-fold brighter than cells expressing ECFP and EYFP(Q69K), respectively.
Changing leucine68 back to valine decreased the relative bleaching time of SYFP2 by 30%. Thus, mutation V68L contributes to the photostability of YFP.