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Comparison List

SCFP3A

SCFP3A is a basic (constitutively fluorescent) cyan fluorescent protein published in 2006, derived from Aequorea victoria. It has low acid sensitivity.

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Aggregation Organism Molecular Weight Cofactor
Monomer Aequorea victoria 26.9 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
433 474 30,000 0.56 16.8 4.5   2.6

Photostability

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SCFP3A Sequence

SCFP3A was derived from SCFP2 with the following mutations: H148D
amino acid numbers relative to avGFP. show relative to SCFP2

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
GenBank: AAZ65848
IPG: 4596895

Structure

Deposited: ,
Chromophore (TWG):

Excerpts

We created monomeric optimized variants of ECFP and EYFP, which fold faster and more efficiently at 37°C and have superior solubility and brightness. Bacteria expressing SCFP3A were 9-fold brighter than those expressing ECFP and 1.2-fold brighter than bacteria expressing Cerulean. In HeLa cells, the improvements were less pronounced; nonetheless, cells expressing SCFP3A and SYFP2 were both 1.5-fold brighter than cells expressing ECFP and EYFP(Q69K), respectively.

Kremers et al. (2006)

Primary Reference

Additional References

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External Resources

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