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|Monomer||Aequorea victoria||26.9 kDa||-|
|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
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SCFP3A was derived from SCFP2 with the following mutations: H148D
amino acid numbers relative to avGFP. show relative to SCFP2
We created monomeric optimized variants of ECFP and EYFP, which fold faster and more efficiently at 37°C and have superior solubility and brightness. Bacteria expressing SCFP3A were 9-fold brighter than those expressing ECFP and 1.2-fold brighter than bacteria expressing Cerulean. In HeLa cells, the improvements were less pronounced; nonetheless, cells expressing SCFP3A and SYFP2 were both 1.5-fold brighter than cells expressing ECFP and EYFP(Q69K), respectively.
Kremers et al. (2006)
(2006). Biochemistry, 45(21) , 6570-6580. doi: 10.1021/bi0516273. Article Pubmed
(2017). Nature Methods, 15(1) , 47-51. doi: 10.1038/nmeth.4509. Article Pubmed
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