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Comparison List

SYFP2

SYFP2 is a basic (constitutively fluorescent) yellow fluorescent protein published in 2006, derived from Aequorea victoria. It has moderate acid sensitivity.
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Aggregation Organism Molecular Weight Cofactor
Monomer Aequorea victoria 26.9 kDa -

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
515 527 101,000 0.68 68.68 6.0   2.9

Photostability

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SYFP2 Sequence

SYFP2 was derived from mVenus with the following mutations: L68V
amino acid numbers relative to avGFP. show relative to mVenus

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
GenBank: DQ092361

Excerpts

Changing leucine68 back to valine decreased the relative bleaching time of SYFP2 by 30%. Thus, mutation V68L contributes to the photostability of YFP.

Kremers et al. (2006)

Primary Reference

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