Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging

Shcherbakova Dm, Baloban M, Emelyanov Av, Brenowitz M, Guo P, Verkhusha Vv

(2016). Nature Communications, 7, 12405. doi: 10.1038/ncomms12405. Article   Pubmed

    Primary Proteins:
  1. miRFP670
  2. miRFP670v1
  3. miRFP703
  4. miRFP709
    Secondary Proteins:
  1. iFP2.0,
  2. RpBphP1

Bleach Measurements

Protein (state) t1/2 (s) Power Light Mode In Cell Fusion ˚C
miRFP670 155.0
miRFP670v1 155.0 200.0 W Arc-lamp Widefield
miRFP670 155.0 200.0 W Arc-lamp Widefield
miRFP703 394.0 200.0 W Arc-lamp Widefield
miRFP709 192.0 200.0 W Arc-lamp Widefield
iRFP682 490.0 200.0 W Arc-lamp Widefield
iRFP702 630.0 200.0 W Arc-lamp Widefield
mIFP 54.0 200.0 W Arc-lamp Widefield
iFP2.0 108.0 200.0 W Arc-lamp Widefield
iRFP670 290.0 200.0 W Arc-lamp Widefield
iRFP713 960.0 200.0 W Arc-lamp Widefield
iRFP720 490.0 200.0 W Arc-lamp Widefield
A caution on interpretation of photostability measurements
Add photostability measurements

Excerpts

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Excerpts are snippets from publications that capture key information about a protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).