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Comparison List

iFP2.0

iFP2.0 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2014, derived from Rhodopseudomonas palustris. It requires the cofactor biliverdin for fluorescence.
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Oligomerization Organism Molecular Weight Cofactor
Dimer Rhodopseudomonas palustris 34.9 kDa Biliverdin

FPbase ID: B53TZ

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
690 711 86,000 0.08 6.88      

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
108.0 200.0 (W) Arc-lamp Widefield HeLa Shcherbakova et al. (2016)

iFP2.0 Sequence

iFP2.0 was derived from iFP1.4 with the following mutations: L6Q/E80D/A87T/P94Q/T168S/M186V/F198Y/H207T/E307Y/K311L/G314L

MARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTADGHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLDWPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQTVRELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLRLTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGGQLWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA

Structure

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for iFP2.0
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Additional References

External Resources

Change history

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