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Comparison List

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iFP2.0

iFP2.0 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2014, derived from Rhodopseudomonas palustris. It requires the cofactor biliverdin for fluorescence.

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Oligomerization	Organism	Molecular Weight	Cofactor
Dimer	Rhodopseudomonas palustris	34.9 kDa	Biliverdin

FPbase ID: B53TZ

Attributes

Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
690	711	86,000	0.08	6.88

Edit States/Attributes

Photostability

t_1/2 (s)	Power	Light	Mode	In Cell	Fusion	˚C	Reference
108.0	200.0 (W)	Arc-lamp	Widefield	HeLa			Shcherbakova et al. (2016)

A caution on interpretation of photostability measurements

Add photostability info

iFP2.0 Sequence

BLAST

iFP2.0 was derived from iFP1.4 with the following mutations: L6Q/E80D/A87T/P94Q/T168S/M186V/F198Y/H207T/E307Y/K311L/G314LL6Q/E80D/A87T/P94Q/T168S/M186V/F198Y/H207T/E307Y/K311L/G314L

MARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTADGHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLDWPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQTVRELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLRLTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGGQLWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA

Structure

PDB ID

Deposited: ,
Chromophore:

Excerpts

No excerpts have been added for iFP2.0
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).