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Comparison List

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miRFP670

This protein has unmoderated changes. Click here to view the last moderated version of this protein from Apr 17, 2019 13:57:57. Or view the full change history

miRFP670 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2016, derived from Rhodopseudomonas palustris. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.

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Oligomerization	Organism	Molecular Weight	Cofactor
Monomer	Rhodopseudomonas palustris	34.5 kDa	Biliverdin

FPbase ID: DP999

Attributes

Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
642	670	87,400	0.14	12.24	4.5	180.0	1.42

Edit States/Attributes

Photostability

t_1/2 (s)	Power	Light	Mode	In Cell	˚C	Reference
155.0						Shcherbakova et al. (2016)
155.0	200.0 (W)	Arc-lamp	Widefield	HeLa		Shcherbakova et al. (2016)
490.0	200.0 (W)	Arc-lamp	Widefield	HeLa	23.0	Matlashov et al. (2020)

A caution on interpretation of photostability measurements

Add photostability info

miRFP670 Sequence

BLAST

miRFP670 was derived from miRFP670v1 with the following mutations: S20CS20C

MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDHRVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVTYQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLVVCHHYLPRFIRFELRAICKRLAERIATRITALES

GenBank: AOD74926

Excerpts

No excerpts have been added for miRFP670
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).