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Comparison List

miRFP709

miRFP709 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2016, derived from Rhodopseudomonas palustris. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Rhodopseudomonas palustris 34.6 kDa Biliverdin

FPbase ID: Q6V69

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
683 709 78,400 0.054 4.23 4.5    
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
192.0 200.0 (W) Arc-lamp Widefield Shcherbakova et al. (2016)
500.0 200.0 (W) Arc-lamp Widefield 23.0 Matlashov et al. (2020)
A caution on interpretation of photostability measurements
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miRFP709 Sequence

miRFP709 was derived from miRFP670 with the following mutations: T201F/V202I/C253I

MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDHRVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSFIPQMARQLYVRQRVRVLVDVTYQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLVVCHHYLPRFIRFELRAICKRLAERIATRITALES

Excerpts

No excerpts have been added for miRFP709
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging

Shcherbakova Dm, Baloban M, Emelyanov Av, Brenowitz M, Guo P, Verkhusha Vv

(2016). Nature Communications, 7, 12405. doi: 10.1038/ncomms12405. Article   Pubmed

Additional References

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