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Comparison List

iRFP682

similar: miRFP682

iRFP682 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2013, derived from Rhodopseudomonas palustris. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
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Oligomerization Organism Molecular Weight Cofactor
Dimer Rhodopseudomonas palustris 34.5 kDa Biliverdin

FPbase ID: N3G27

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
663 682 90,000 0.11 9.9 4.5   0.95
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
490.0 200.0 (W) Arc-lamp Widefield HeLa Shcherbakova et al. (2016)
A caution on interpretation of photostability measurements
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iRFP682 Sequence

BLAST

iRFP682 was derived from iRFP713 with the following mutations: E180V/T202A/V254C

MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGSDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGVVIAEDRCAEVESKLGLHYPASAVPAQARRLYTINPVRIIPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPCHLEFMRNIGMHGTMSISILRGERLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE
GenBank: AGN32864

Excerpts

No excerpts have been added for iRFP682
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Near-infrared fluorescent proteins for multicolor in vivo imaging

Shcherbakova Dm, Verkhusha Vv

(2013). Nature Methods, 10(8) , 751-754. doi: 10.1038/nmeth.2521. Article

Additional References

  1. Time-resolved flow cytometry for lifetime measurements of near-infrared fluorescent proteins

    Yang Z, Shcherbakova Dm, Verkhusha V, Houston Jp

    (2016). Conference on Lasers and Electro-Optics, , . doi: 10.1364/cleo_si.2016.sw4g.1. Article

External Resources

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