compare

Comparison List

miRFP703

miRFP703 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2016, derived from Rhodopseudomonas palustris. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
+
Oligomerization Organism Molecular Weight Cofactor
Monomer Rhodopseudomonas palustris 34.5 kDa Biliverdin

FPbase ID: 1B7TK

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
674 703 90,900 0.086 7.82 4.5    
Edit States/Attributes

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
394.0 200.0 (W) Arc-lamp Widefield HeLa Shcherbakova et al. (2016)
650.0 200.0 (W) Arc-lamp Widefield HeLa 23.0 Matlashov et al. (2020)
A caution on interpretation of photostability measurements
Add photostability info

miRFP703 Sequence

BLAST

miRFP703 was derived from miRFP670 with the following mutations: T201L/C253I

MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDHRVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVTYQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLVVCHHYLPRFIRFELRAICKRLAERIATRITALES

Excerpts

No excerpts have been added for miRFP703
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging

Shcherbakova Dm, Baloban M, Emelyanov Av, Brenowitz M, Guo P, Verkhusha Vv

(2016). Nature Communications, 7, 12405. doi: 10.1038/ncomms12405. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

Change history

Something missing or incorrect? Submit a change Submit a change