Comparison List


a.k.a. iRFP

similar: miRFP713

iRFP713 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2011, derived from Rhodopseudomonas palustris. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
Oligomerization Organism Molecular Weight Cofactor
Dimer Rhodopseudomonas palustris 34.6 kDa Biliverdin



Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
690 713 105,000 0.06 6.3 4.0 168.0  


t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
450.0   Filonov et al. (2011)
960.0 200.0 (W) Arc-lamp Widefield HeLa Shcherbakova et al. (2016)

iRFP713 Sequence

iRFP713 was derived from RpBphP2 with the following mutations: T2A/S13L/A92T/V104I/V114I/E161K/Y193K/F198Y/D202T/I203V/Y258F/A283V/K288T/N290Y

GenBank: AEL88490


iRFP is stable, noncytotoxic and the low concentrations of endogenous BV [in mammalian cells] are sufficient to make it brightly fluorescent in cells, tissues and whole animals. These features make its application as easy as the conventional GFP-like fluorescent proteins and hence should broaden the possibilities of noninvasive in vivo imaging

Filonov et al. (2011)

Primary Reference

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