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iRFP713

a.k.a. iRFP

iRFP713 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2011, derived from Rhodopseudomonas palustris. It is reported to be a very slowly-maturing dimer with low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
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Aggregation Organism Molecular Weight Cofactor
Dimer Rhodopseudomonas palustris 34.6 kDa Biliverdin

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
690 713 105,000 0.06 6.3 4.0 168.0  

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
450.0 Filonov et al. (2011)

iRFP713 Sequence

iRFP713 was derived from RpBphP2 with the following mutations: T2A/S13L/A92T/V104I/V114I/E161K/Y193K/F198Y/D202T/I203V/Y258F/A283V/K288T/N290Y

MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGSDNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKITGFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDINYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGLIVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE
GenBank: AEL88490

Excerpts

iRFP is stable, noncytotoxic and the low concentrations of endogenous BV [in mammalian cells] are sufficient to make it brightly fluorescent in cells, tissues and whole animals. These features make its application as easy as the conventional GFP-like fluorescent proteins and hence should broaden the possibilities of noninvasive in vivo imaging

Filonov et al. (2011)

Primary Reference

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