a.k.a. iRFP
similar: miRFP713
| Oligomerization | Organism | Molecular Weight | Cofactor |
|---|---|---|---|
| Dimer | Rhodopseudomonas palustris | 34.6 kDa | Biliverdin |
| Ex λ | Em λ | EC (M-1 cm-1) | QY | Brightness | pKa | Maturation (min) | Lifetime (ns) |
|---|---|---|---|---|---|---|---|
| 690 | 713 | 105,000 | 0.06 | 6.3 | 4.0 | 168.0 |
| t1/2 (s) | Power | Light | Mode | In Cell | Fusion | ˚C | Reference |
|---|---|---|---|---|---|---|---|
| 450.0 | Filonov et al. (2011) | ||||||
| 960.0 | 200.0 (W) | Arc-lamp | Widefield | HeLa | Shcherbakova et al. (2016) |
iRFP713 was derived from RpBphP2 with the following mutations: T2A/S13L/A92T/V104I/V114I/E161K/Y193K/F198Y/D202T/I203V/Y258F/A283V/K288T/N290Y
iRFP is stable, noncytotoxic and the low concentrations of endogenous BV [in mammalian cells] are sufficient to make it brightly fluorescent in cells, tissues and whole animals. These features make its application as easy as the conventional GFP-like fluorescent proteins and hence should broaden the possibilities of noninvasive in vivo imaging
Filonov et al. (2011)
(2011). Nature Biotechnology, 29(8) , 757-761. doi: 10.1038/nbt.1918. Article Pubmed
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