compare

Comparison List

miRFP670v1

miRFP670v1 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2016, derived from Rhodopseudomonas palustris. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.

No spectrum has been submitted ... add a spectrum!

Oligomerization Organism Molecular Weight Cofactor
Monomer Rhodopseudomonas palustris 34.5 kDa Biliverdin

FPbase ID: GCOU1

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
644 670 71,300 0.116 8.27 4.5    
Edit States/Attributes

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
155.0 200.0 (W) Arc-lamp Widefield HeLa Shcherbakova et al. (2016)
A caution on interpretation of photostability measurements
Add photostability info

miRFP670v1 Sequence

BLAST

miRFP670v1 was derived from RpBphP1 with the following mutations: D16S/L17H/C20S/R22H/P43H/I47V/D106C/P127S/A155V/E179L/R184C/D201T/I202V/R208Q/E211V/S222T/I253C/Y257F/N260D/V268A/A282V/H292R/E300K/L301R/A305R

MVAGHASGSPAFGTASHSNSEHEEIHLAGSIQPHGALLVVSEHDHRVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVTYQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLVVCHHYLPRFIRFELRAICKRLAERIATRITALES

Excerpts

No excerpts have been added for miRFP670v1
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging

Shcherbakova Dm, Baloban M, Emelyanov Av, Brenowitz M, Guo P, Verkhusha Vv

(2016). Nature Communications, 7, 12405. doi: 10.1038/ncomms12405. Article   Pubmed

Additional References

    No additional references have been added.

External Resources

Change history

Something missing or incorrect? Submit a change Submit a change