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Comparison List

miRFP670v1

miRFP670v1 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2016, derived from Rhodopseudomonas palustris. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Rhodopseudomonas palustris 34.5 kDa Biliverdin

FPbase ID: GCOU1

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
644 670 71,300 0.116 8.27 4.5    

Photostability

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miRFP670v1 Sequence

miRFP670v1 was derived from RpBphP1 with the following mutations: D16S/L17H/C20S/R22H/P43H/I47V/D106C/P127S/A155V/E179L/R184C/D201T/I202V/R208Q/E211V/S222T/I253C/Y257F/N260D/V268A/A282V/H292R/E300K/L301R/A305R

MVAGHASGSPAFGTASHSNSEHEEIHLAGSIQPHGALLVVSEHDHRVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVTYQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLVVCHHYLPRFIRFELRAICKRLAERIATRITALES

Excerpts

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Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Additional References

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External Resources

Change history

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