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Comparison List

iRFP702

similar: miRFP702

iRFP702 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2013, derived from Rhodopseudomonas palustris CGA009. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
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Oligomerization Organism Molecular Weight Cofactor
Dimer Rhodopseudomonas palustris CGA009 34.5 kDa Biliverdin

FPbase ID: 7WEWT

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
673 702 93,000 0.08 7.44 4.5    
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Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
630.0 200.0 (W) Arc-lamp Widefield Shcherbakova et al. (2016)
A caution on interpretation of photostability measurements
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iRFP702 Sequence

iRFP702 was derived from RpBphP6 with the following mutations: P2A/S41T/D120E/M164L/D196L/I197V/I213V/R227H/V244I/Y251F/A276I/F300L

MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAGAFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLHRHDGTSIVEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVMLYRFADDGSGKVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSRIVPEHDASGAALDLSFAHLRSISPIHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYEPRAVPMAQRVAAEMFADFLSLHFTAAHHQR
GenBank: AGN32865

Excerpts

No excerpts have been added for iRFP702
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Near-infrared fluorescent proteins for multicolor in vivo imaging

Shcherbakova Dm, Verkhusha Vv

(2013). Nature Methods, 10(8) , 751-754. doi: 10.1038/nmeth.2521. Article

Additional References

    No additional references have been added.

External Resources

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