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Comparison List

iRFP670

similar: miRFP670

iRFP670 is a basic (constitutively fluorescent) near ir fluorescent protein published in 2013, derived from Rhodopseudomonas palustris CGA009. It has low acid sensitivity. It requires the cofactor biliverdin for fluorescence.
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Oligomerization Organism Molecular Weight Cofactor
Dimer Rhodopseudomonas palustris CGA009 34.5 kDa Biliverdin

FPbase ID: Q5DYR

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
643 670 114,000 0.11 12.54 4.0    

Photostability

t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
290.0 200.0 (W) Arc-lamp Widefield HeLa Shcherbakova et al. (2016)

iRFP670 Sequence

iRFP670 was derived from iRFP702 with the following mutations: V112I/K174M/I247C

MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAGAFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLHRHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVMLYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSRIVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYEPRAVPMAQRVAAEMFADFLSLHFTAAHHQR
GenBank: AGN32863

Excerpts

No excerpts have been added for iRFP670
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Near-infrared fluorescent proteins for multicolor in vivo imaging

Shcherbakova Dm, Verkhusha Vv

(2013). Nature Methods, 10(8) , 751-754. doi: 10.1038/nmeth.2521. Article

Additional References

  1. Two-Photon Excitation Spectra of Various Fluorescent Proteins within a Broad Excitation Range

    Leben R, Lindquist Rl, Hauser Ae, Niesner R, Rakhymzhan A

    (2022). International Journal of Molecular Sciences, 23(21) , 13407. doi: 10.3390/ijms232113407. Article

External Resources

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