Comparison List


mRuby3 is a basic (constitutively fluorescent) red fluorescent protein published in 2016, derived from Entacmaea quadricolor. It has low acid sensitivity.
Oligomerization Organism Molecular Weight Cofactor
Monomer Entacmaea quadricolor 26.6 kDa -



Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
558 592 128,000 0.45 57.6 4.8 136.5  

mRuby3 OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
14.0 (70 cells) - U-2 OS Bindels et al. (2016)


t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
349.0   Bajar et al. (2016)

mRuby3 Sequence

mRuby3 was derived from mRuby2 with the following mutations: N33R/M36E/T38V/K74A/G75D/M105T/C114E/H118N/Q120K/H159D/M160I/S171H/S173N/I192V/L202I/M209T/F210Y/H216V/F221Y/A222S/G223N
amino acid numbers relative to eqFP611. show relative to mRuby2

GenBank: ATE88097


Negligible photochromic behavior was measured for the mScarlet variants, while TagRFP-T, mRuby2, mRuby3 and mApple showed 15%, 19%, 41%, and 51% photochromic behavior, respectively. Hence, extreme care must be taken when using the latter four RFP variants as acceptors in FRET studies, since a photochromic effect is easily confused with a changed FRET state, especially if one considers that the typical FRET contrast in many sensors is in the range of only 5–20%. The photochromic behavior can also interfere with characterization of FPs, like determination of photostability (Supplementary Fig. 8) or brightness (Supplementary Fig. 5l).

Bindels et al. (2016)

Primary Reference

Additional References

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