Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting

Bajar Bt, Wang Es, Lam Aj, Kim Bb, Jacobs Cl, Howe Es, Davidson Mw, Lin Mz, Chu J

(2016). Scientific Reports, 6(1) , 20889. doi: 10.1038/srep20889. Article   Pubmed

    Primary Proteins:
  1. Clover1.5
  2. dClover2
  3. dClover2 A206K
  4. mClover1.5
  5. mClover3
  6. mRuby3

Bleach Measurements

Protein (state) t1/2 (s) Power Light Mode In Cell Fusion ˚C
mClover3 80.0
mRuby3 349.0
A caution on interpretation of photostability measurements
Add photostability measurements

Excerpts

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