Comparing the performance of mScarlet-I, mRuby3, and mCherry as FRET acceptors for mNeonGreen

Mccullock Tw, Maclean Dm, Kammermeier Pj

(2020). PLOS ONE, 15(2) , e0219886. doi: 10.1371/journal.pone.0219886. Article   Pubmed

    Secondary Proteins:
  1. mCherry,
  2. mClover3,
  3. mNeonGreen,
  4. mRuby3,
  5. mScarlet,
  6. mScarlet-I
Add photostability measurements

Excerpts

Overall, we find that mNeonGreen is an excellent FRET donor, and both mCherry and mScarlet-I, but not mRuby3, act as practical FRET acceptors, with the brighter mScarlet-I out performing mCherry in intensiometric studies, but mCherry out performing mScarlet-I in instances where consistent efficiency in a population is critical.