|Protein||% Normal Cells||OSER/NE ratio||Cell Type||Temp (˚C)|
|mAvicFP1||66.0 (517 cells)||2.31 ± 0.915 (517 cells)||U2-OS||-|
AausFP4 is the first natural example of Dreiklang-type photochromism, and may help generate other useful variations on this mechanism.
When expressed in total darkness, AvicFP2 is weakly green fluorescent with spectra suggesting an avGFP-type chromophore. After blue light exposure, AvicFP2 rapidly converts (within seconds to minutes), to a purple-blue CP (peak absorbance at 588 nm) which is completely non-fluorescent.
AvicFP3 is highly homologous to AvicFP2 (96% amino acid identity), and is similarly green fluorescent when expressed and purified in the dark. Like AvicFP2, AvicFP3 converts to a green-absorbing chromoprotein when exposed to blue light. We suspect that AvicFP3 is so highly sensitive to blue light exposure that the act of taking an absorbance measurement is enough to partially photoconvert the protein.