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Comparison List

AvicFP2

AvicFP2 is a basic (constitutively fluorescent) green fluorescent protein published in 2019, derived from Aequorea victoria.
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Oligomerization Organism Molecular Weight Cofactor
? Aequorea victoria 25.6 kDa -

FPbase ID: FHG23

Attributes

State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
post-conversion     41,000          
pre-conversion 480 515 59,000 0.04 2.36      

Transitions

From To Switch λ
pre-conversion post-conversion 480

Photostability

No photostability measurements available ... add one!

AvicFP2 Sequence

MNCGALLFKQKIPFIVELDGDVNGQVFSVRGNGFGDGSIGKIEIKFVCTTGEIPVAWESLVCSLAYGALVFTKYPSNINDFFKSTMPKGYIQERKITFENDGQYETRAEVTYENGALYNRVKLNGSGFRKDGNILGKKLDATSLGTCIYILSNDEGNGLKGVYNMAYKVIDGNRQIVSHQQTNTPIGDGLVSIPDYHVMHNHISCSKDPTEPRDHLILKESVTAVDCQTAYM

Excerpts

When expressed in total darkness, AvicFP2 is weakly green fluorescent with spectra suggesting an avGFP-type chromophore. After blue light exposure, AvicFP2 rapidly converts (within seconds to minutes), to a purple-blue CP (peak absorbance at 588 nm) which is completely non-fluorescent.

Lambert et al. (2019)

Primary Reference

Additional References

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External Resources

Change history

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