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Comparison List

mAvicFP1

similar: AvicFP1

mAvicFP1 is a basic (constitutively fluorescent) green fluorescent protein published in 2019, derived from Aequorea victoria. It has low acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Aequorea victoria 26.8 kDa -

FPbase ID: SXYKQ

Attributes

Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
480 503 65,000 0.63 40.95 4.9    
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mAvicFP1 OSER Measurements

% Normal Cells OSER/NE ratio Cell Type Reference
66.0 (517 cells) 2.31 ± 0.915 (517 cells) U2-OS Lambert et al. (2019)

Photostability

No photostability measurements available ... add one!

mAvicFP1 Sequence

mAvicFP1 was derived from AvicFP1 with the following mutations: F64L/A206K

MSKGAELFNGIVPILIELNGDVHGHKFSVRGEGEGDAGSGKIEIKFVCTTGTLPVPWPTLVTTLCYGVQCFTRYPEHMKQHDFYKSAMPDGYIQKRTISFQDDGHYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGNKMEYNYNSHSVYVLSDKANNGIKVNFKIRHNLKGEGIQLADHDQQNIPIGDGPVLLPDYHYLSTQTKITKDPNEKRDHMNLVEFVTACGITHGMDELYK

Excerpts

As an apparently superior scaffold to avGFP in many ways, mAvicFP1 may be quickly adaptable to existing probes and biosensors

Lambert et al. (2019)

Primary Reference

Aequorea victoria’s secrets

Lambert Gg, Depernet H, Gotthard G, Schultz Dt, Navizet I, Lambert T, Bindels Ds, Levesque V, Moffatt Jn, Salih A, Royant A, Shaner Nc

(2019). , , . doi: 10.1101/677344. Article

Additional References

  1. Live-cell nanoscopy with spontaneous blinking of conventional green fluorescent proteins

    Gavrikov As, Baranov Ms, Mishin As

    (2020). Biochemical and Biophysical Research Communications, 522(4) , 852-854. doi: 10.1016/j.bbrc.2019.11.163. Article   Pubmed

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