Primary Proteins:
  1. dLanYFP
  2. LanYFP
  3. mNeonGreen
    Secondary Proteins:
  1. Clover,
  2. mTurquoise2

Bleach Measurements

Protein (state) t1/2 (s) Power Light Mode In Cell Fusion ˚C
Clover 50.0
mNeonGreen 158.0 4.3 mW Arc-lamp Widefield H2B
A caution on interpretation of photostability measurements
Add photostability measurements

OSER Measurements

Protein % Normal Cells OSER/NE ratio Cell Type Temp (˚C)
mEGFP 98.1 ± 1.6 (10000 cells) - HeLa -
mTurquoise 93.8 ± 1.0 (10000 cells) - HeLa -
mNeonGreen 90.4 ± 2.1 (10000 cells) - HeLa -
mRuby2 87.4 ± 5.8 (10000 cells) - HeLa -
EGFP 76.5 ± 6.9 (10000 cells) - HeLa -
Clover 72.9 ± 5.5 (10000 cells) - HeLa -

Excerpts

mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.

... as it has so little sequence identity in common with other frequently used fluorescent proteins, mNeonGreen will be an attractive target for antibody development and should be amenable to orthogonal immunoprecipitation experiments along with jellyfish-derived and coral-derived fluorescent proteins.

Most newly cloned green and yellow fluorescent proteins are not subjected to additional engineering simply because of their lack of improved properties relative to existing proteins. Thus, we were intrigued that a yellow fluorescent protein from B. lanceolatum (LanYFP) exhibits an unusually high quantum yield (∼0.95) and extinction coefficient (∼150,000 M−1 cm−1), making it a very attractive candidate for additional development.