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Most newly cloned green and yellow fluorescent proteins are not subjected to additional engineering simply because of their lack of improved properties relative to existing proteins. Thus, we were intrigued that a yellow fluorescent protein from B. lanceolatum (LanYFP) exhibits an unusually high quantum yield (∼0.95) and extinction coefficient (∼150,000 M−1 cm−1), making it a very attractive candidate for additional development.