Excerpts

Rosmarinus remains tetrameric and becomes dimeric only at nanomolar concentrations (∼70 nM)... Rosmarinus was made using PCR and the In-Fusion cloning technique (Clontech) to fuse the front half of the coding region for mc5 to the back half of meleCFP at a five amino acid identical stretch in their coding sequences.

Table 1: Fluorescence quantum yield of amFP486: 0.75 (0.24 (Matz et al., 1999), 0.71 (Henderson et al., 2005))