Primary Proteins:
  1. Rosmarinus
    Secondary Proteins:
  1. amFP486,
  2. dsFP483,
  3. dTFP0.2,
  4. EGFP,
  5. KCY-G4219,
  6. meffCFP,
  7. meleCFP,
  8. mNeonGreen
Add photostability measurements


Rosmarinus remains tetrameric and becomes dimeric only at nanomolar concentrations (∼70 nM)... Rosmarinus was made using PCR and the In-Fusion cloning technique (Clontech) to fuse the front half of the coding region for mc5 to the back half of meleCFP at a five amino acid identical stretch in their coding sequences.

Table 1: Fluorescence quantum yield of amFP486: 0.75 (0.24 (Matz et al., 1999), 0.71 (Henderson et al., 2005))