Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation

Molina Rs, Tran Tm, Campbell Re, Lambert Gg, Salih A, Shaner Nc, Hughes Te, Drobizhev M

(2017). The Journal of Physical Chemistry Letters, 8(12) , 2548-2554. doi: 10.1021/acs.jpclett.7b00960. Article   Pubmed

    Primary Proteins:
  1. Rosmarinus
    Secondary Proteins:
  1. amFP486,
  2. dsFP483,
  3. dTFP0.2,
  4. EGFP,
  5. KCY-G4219,
  6. meffCFP,
  7. meleCFP,
  8. mNeonGreen
Add photostability measurements

Excerpts

Rosmarinus remains tetrameric and becomes dimeric only at nanomolar concentrations (∼70 nM)... Rosmarinus was made using PCR and the In-Fusion cloning technique (Clontech) to fuse the front half of the coding region for mc5 to the back half of meleCFP at a five amino acid identical stretch in their coding sequences.