|State||Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
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... our results show that mMaple has a much higher signaling efficiency than Dendra2, mEos2, mEos3.2, tdEos, mKikGR, PAmCherry, and PATagRFP. However, the dimerization tendency of mMaple could lead to aggregation effects on the target proteins. Dendra2, mEos3.2, and PATagRFP exhibit undetectable dimerization tendency, but have low signaling efficiency. It is thus desirable to develop a new PAFP that has both high signaling efficiency and low dimerization tendency. To this end, we engineered two new PAFPs by introducing point mutations into mMaple designed to destabilize the dimerization of this protein.