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Comparison List

mEos3.2

mEos3.2 is a photoconvertible green/yellow fluorescent protein published in 2012, derived from Lobophyllia hemprichii. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity.
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Oligomerization Organism Molecular Weight Cofactor
Monomer Lobophyllia hemprichii 25.7 kDa -

FPbase ID: VUXRF

Attributes

State Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns)
Green 507 516 63,400 0.84 53.26 5.4 20.0  
Red 572 580 32,200 0.55 17.71 5.8    

Transitions

From To Switch λ
Green Red 405

Photostability

State t1/2 (s) Power Light Mode In Cell Fusion ˚C Reference
Green 12.55 24.0 (µW) Laser Point Scanning Confocal H2B Zhang et al. (2012)
Red 48.0 64.0 (µW) Laser Point Scanning Confocal H2B Zhang et al. (2012)

mEos3.2 Sequence

mEos3.2 was derived from mEos2-NA with the following mutations: H158E

MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKEGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNARNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLLEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDNARR

Excerpts

No excerpts have been added for mEos3.2
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Additional References

External Resources

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