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Comparison List

mClavGR1

mClavGR1 is a fluorescent protein published in 2010, derived from Clavularia sp.. It is reported to be a monomer.

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Oligomerization Organism Molecular Weight Cofactor
Monomer Clavularia sp. 27.0 kDa -

FPbase ID: 9BUDR

Attributes

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Photostability

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mClavGR1 Sequence

mClavGR1 was derived from mTFP1 with the following mutations: G44S/K55R/E72S/Y76F/D77E/N80Q/N83D/S95A/A104H/A109V/N119D/T134S/K140G/V143C/K144I/V145A/K146T/S147N/S150T/E153K/Y158N/E159K/L162F/T179V/D182E/R187K/H201M/E206K/H211Y/V213C/K216R/I218T/R220K/K222_K223insQ/N241S/T250K/V251L/S254H/R258H/N259S/S260G/T261L/D261aP
amino acid numbers relative to cFP484. show relative to mTFP1

Note: Note: Supp. Table 1 in Hoi et al (2010) does not list mTFP1-T141V (position relative to mTFP1 as shown in paper) as one of the mutations in mClavGR1. But Fig. 2 in the paper shows all mClavGR proteins with 141V. It is included in the sequence and mutation list here (as T146V relative to mTFP1).

MVSKGEETTMSVIKPDMKIKLRMEGNVNGHAFVIEGEGSGKPFEGTQTIDLEVKEGAPLPFAYDILTTAFHYGNRVFTKYPDDIPDYFKQSFPEGYSWERSMTFEDGGICIATNDITMEKDSFINKIHFKGENFPPNGPVMQKKTVGWEASTEKMYVRDGVLKGDVKMKLLLKGGGHYRCDFRTTYKAKQKAVKLPDYHFVDHRIEILSHDKDYNKVKLYEHAVAHSGLPGMDELYK

Excerpts

The new photoconvertible FP based on the mTFP*1 template was designed using a strategy analogous to that previously used to design a consensus FP based on a monomeric Azami green template.25 The known photoconvertible FPs, including EosFP, Dendra2, KikGR, and Kaede were aligned to find the consensus at each amino acid position. Amino acids of > 50% consensus were maintained in the designed protein. At positions with no clear consensus, the corresponding residue in mTFP*1 was used. Residues of mTFP*1 that had been substituted during the conversion of the wild-type cFP484 tetramer into a monomer were maintained in the design of the consensus protein

Hoi et al. (2010)

Primary Reference

A Monomeric Photoconvertible Fluorescent Protein for Imaging of Dynamic Protein Localization

Hoi H, Shaner Nc, Davidson Mw, Cairo Cw, Wang J, Campbell Re

(2010). Journal of Molecular Biology, 401(5) , 776-791. doi: 10.1016/j.jmb.2010.06.056. Article   Pubmed

Additional References

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Change history

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