A Monomeric Photoconvertible Fluorescent Protein for Imaging of Dynamic Protein Localization

Hoi H, Shaner Nc, Davidson Mw, Cairo Cw, Wang J, Campbell Re

(2010). Journal of Molecular Biology, 401(5) , 776-791. doi: 10.1016/j.jmb.2010.06.056. Article   Pubmed

    Primary Proteins:
  1. dClavGR1.6
  2. mClavGR1
  3. mClavGR1.1
  4. mClavGR1.8
  5. mClavGR2
Add photostability measurements


The new photoconvertible FP based on the mTFP*1 template was designed using a strategy analogous to that previously used to design a consensus FP based on a monomeric Azami green template.25 The known photoconvertible FPs, including EosFP, Dendra2, KikGR, and Kaede were aligned to find the consensus at each amino acid position. Amino acids of > 50% consensus were maintained in the designed protein. At positions with no clear consensus, the corresponding residue in mTFP*1 was used. Residues of mTFP*1 that had been substituted during the conversion of the wild-type cFP484 tetramer into a monomer were maintained in the design of the consensus protein