(2022). Scientific Reports, 12(1) , 10190. doi: 10.1038/s41598-022-13214-0. Article Pubmed
Protein (state) | t1/2 (s) | Power | Light | Mode | In Cell | Fusion | ˚C |
---|---|---|---|---|---|---|---|
Electra1 | 950.0 | 4.25 mW/mm2 | LED | Widefield | 21.0 | ||
Electra1 | 2124.0 | 0.91 mW/mm2 | LED | Widefield | 21.0 | ||
Electra1 | 185.0 | 3.72 mW/mm2 | LED | Widefield | 21.0 | ||
Electra2 | 610.0 | 4.25 mW/mm2 | LED | Widefield | 21.0 | ||
Electra2 | 1466.0 | 0.91 mW/mm2 | LED | Widefield | 21.0 | ||
Electra2 | 200.0 | 3.72 mW/mm2 | LED | Widefield | 21.0 | ||
mBlueberry2 | 710.0 | 4.25 mW/mm2 | LED | Widefield | 21.0 | ||
mBlueberry2 | 25.0 | 0.91 mW/mm2 | LED | Widefield | 21.0 | ||
mTagBFP2 | 1150.0 | 4.25 mW/mm2 | LED | Widefield | 21.0 | ||
mTagBFP2 | 996.0 | 0.91 mW/mm2 | LED | Widefield | 21.0 | ||
mTagBFP2 | 135.0 | 3.72 mW/mm2 | LED | Widefield | 21.0 | ||
EBFP2 | 635.0 | 3.72 mW/mm2 | LED | Widefield | 21.0 | ||
EBFP2 | 1080.0 | 4.25 mW/mm2 | LED | Widefield | 21.0 |
Quantification of intracellular brightness revealed that Electra1 and Electra2 were 2.3- and 2.1-fold brighter than mTagBFP2
When transiently expressed in HEK cells, Electras were more than fourfold brighter than mBlueberry2 and about 1.4-fold brighter than EBFP2, while slightly dimmer than mTagBFP2 (Fig. 3a, Table 1).