An Engineered Monomeric Zoanthus sp. Yellow Fluorescent Protein

Hoi H, Howe Es, Ding Y, Zhang W, Baird Ma, Sell Br, Allen Jr, Davidson Mw, Campbell Re

(2013). Chemistry & Biology, 20(10) , 1296-1304. doi: 10.1016/j.chembiol.2013.08.008. Article   Pubmed

    Primary Proteins:
  1. dPapaya0.1
  2. mPapaya
  3. mPapaya0.3
  4. mPapaya0.6
  5. mPapaya0.7
  6. tPapaya0.01
Add photostability measurements

OSER Measurements

Protein % Normal Cells OSER/NE ratio Cell Type Temp (˚C)
mPapaya 87.6 ± 3.2 (10000 cells) - HeLa -
mCitrine 93.8 ± 2.6 (10000 cells) - HeLa -
mPapaya0.6 95.1 ± 1.1 (10000 cells) - HeLa -
EGFP 76.5 ± 6.9 (10000 cells) - HeLa -
mVenus 83.9 ± 4.4 (10000 cells) - HeLa -

Excerpts

A drawback of mPapaya0.6 that soon became apparent was relatively poor photostability under typical live cell imaging conditions. Similar to mTFP0.7, photobleached mPapaya0.6 displayed a 410 nm absorbance peak attributable to the protonated state of the chromophore and was observed to recover fluorescence when stored in the dark