Bright monomeric red fluorescent protein with an extended fluorescence lifetime

Merzlyak Em, Goedhart J, Shcherbo D, Bulina Me, Shcheglov As, Fradkov Af, Gaintzeva A, Lukyanov Ka, Lukyanov S, Gadella Twj, Chudakov Dm

(2007). Nature Methods, 4(7) , 555-557. doi: 10.1038/nmeth1062. Article   Pubmed

    Primary Proteins:
  1. eqFP578
  2. TagRFP
  3. TurboRFP
    Secondary Proteins:
  1. eqFP611,
  2. mCherry,
  3. TurboFP602

Bleach Measurements

Protein (state) t1/2 (s) Power Light Mode In Cell Fusion ˚C
TagRFP 48.0
A caution on interpretation of photostability measurements
Add photostability measurements

Excerpts

Together, eqFP578 and eqFP611 represent a separate group of red fluorescent proteins, which are characterized by high fluorescence brightness, complete chromophore maturation and, notably, a reduced tendency to oligomerize. These features make E. quadricolor fluorescent proteins an attractive starting point for the generation of bright monomeric red fluorescent proteins.

Together, eqFP578 and eqFP611 represent a separate group of red fluorescent proteins, which are characterized by high fluorescence brightness, complete chromophore maturation and, notably, a reduced tendency to oligomerize. These features make E. quadricolor fluorescent proteins an attractive starting point for the generation of bright monomeric red fluorescent proteins.