|Ex λ||Em λ||EC (M-1 cm-1)||QY||Brightness||pKa||Maturation (min)||Lifetime (ns)|
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The bacterial cytotoxicity assay demonstrated that RDSmCherry1 has cytotoxicity similar to that of mCherry and LSSmCherry1, indicating that the decreased cytotoxicity of mCherry2 was lost during directed evolution.
... We have used protein design and directed evolution to develop new RFP variants with low cytotoxicity to E. coli and altered fluorescence excitation and emission profiles. However, at this stage of development, none of these variants are improved relative to the current state of the art monomeric RFPs in their respective classes. ... mCardinal, which is derived from Entacmaea quadricolor eqFP578, is 3.3× brighter than RDSmCherry1 and has an emission peak that is 29 nm more red-shifted. So, while these new mCherry variants are unlikely to find immediate use in live cell imaging applications, they do provide insight into the influence of the protein structure on mCherry’s fluorescence and cytotoxicity properties and may serve as starting points for future probe development efforts.