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Comparison List

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LSSmCherry1

This protein has unmoderated changes. Click here to view the last moderated version of this protein from Oct 9, 2019 08:38:47. Or view the full change history

LSSmCherry1 is a basic (constitutively fluorescent) long stokes shift fluorescent protein published in 2017, derived from Discosoma sp.. It has high acid sensitivity.

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Oligomerization	Organism	Molecular Weight	Cofactor
Monomer	Discosoma sp.	26.7 kDa	-

FPbase ID: EY2GX

Attributes

Ex λ	Em λ	EC (M^-1 cm^-1)	QY	Brightness	pKa	Maturation (min)	Lifetime (ns)
450	610	35,000	0.29	10.15	6.2

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Photostability

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LSSmCherry1 Sequence

BLAST

LSSmCherry1 was derived from mCherry with the following mutations: A11T/K97N/K143C/K144R/W148L/S152T/I166S/Q168E/N201D/T207L/A222SA6bT/K92N/K138C/K139R/W143L/S147T/I161S/Q163E/N196D/T202L/A217S
amino acid numbers relative to mCherryDsRed. show relative to DsRed show relative to mCherry

MVSKGEEDNMTIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGLEASTERMYPEDGALKGESKERLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERSEGRHSTGGMDELYK

Excerpts

No excerpts have been added for LSSmCherry1
Excerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation).

Primary Reference

Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity

Shen Y, Chen Y, Wu J, Shaner Nc, Campbell Re

(2017). PLOS ONE, 12(2) , e0171257. doi: 10.1371/journal.pone.0171257. Article Pubmed

Additional References

No additional references have been added.

External Resources

Search Addgene for LSSmCherry1
Search RIKEN BRC for LSSmCherry1

Change history

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